At 24 h after transduction, total cell lysates were prepared and subjected to SDS-10% PAGE, followed by immunoblotting with anti-SAMHD1 antibody. loss at late stages of computer virus illness. Knockdown of CUL2 and to a lesser degree CUL1 using siRNA … Continue reading At 24 h after transduction, total cell lysates were prepared and subjected to SDS-10% PAGE, followed by immunoblotting with anti-SAMHD1 antibody